The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.
Identifieur interne : 000157 ( Ncbi/Merge ); précédent : 000156; suivant : 000158The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.
Auteurs : Jarosław Przetakiewicz [Pologne] ; Anna Nadolska-Orczyk ; Wacław OrczykSource :
- Cellular & molecular biology letters [ 1425-8153 ] ; 2002.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA Primers.
- genetics : Solanum tuberosum.
- Base Sequence, Diploidy, Genetic Markers, Hybridization, Genetic, Random Amplified Polymorphic DNA Technique.
Abstract
Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.
PubMed: 12378226
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